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Inhibition Studies of Porphobilinogen Synthase fromEscherichia coli usingDiacids

Caroline Jarret, Janette Bobálová, Frédéric Stauffer, Matthias E. Henz and Reinhard Neier*

E-mail:neier.echet98@ich.unine.ch

Abstract

1. Introduction  

1.1. Porphobilinogen synthase: still a mechanistic enigma

1.2. Biochemical aspects

1.3. Site selective inhibition studies

1.4. Kinetics of the inhibition studies of PBGS

1.5. Diacids as analogues of the intermediates

1.6. Inhibition studies of PBGS using diacids reported by other groups

1.7. Choice of diacids tested as inhibitors

2. Experimental Procedures

2.1. Materials

2.2. PBGS assay and determination of kinetic constants

2.3. Estimation of the specific activity

2.4. Dialysis assay

3. Results

3.1. Inhibition of E. coli PBGS with diacids

4. Discussion

5. Conclusion

6. Acknowledgements

7. References